3 Lab 4 Histology

Activity 4.1

Histology Slide Identification and Labeling

Background

Histology is the study of the microscopic structure of tissues. Understanding the various types of epithelial tissues and their locations in the body is crucial for students in the health, medical and biological sciences. This lab activity will focus on identifying and labeling different types of epithelial and connective tissues, particularly in relation to slides of blood vessels, kidney structures, and various other organ tissues.

 

Example of key terms you should be familiar with:

 

Simple Squamous Epithelium: A single layer of flat cells, found in areas where diffusion and filtration occur, such as alveoli and capillaries.

Tunica Intima: The innermost layer of blood vessels, which includes endothelial cells.

Tunica Media: The middle layer of blood vessels, primarily composed of smooth muscle and elastic fibers.

Tunica Adventitia: The outermost layer of blood vessels, providing structural support and protection.

Renal Corpuscle: The filtering unit of the kidney, consisting of the glomerulus and Bowman’s capsule.

Goblet Cells: Cells that secrete mucus, found in the lining of the intestines and respiratory tract.

Transitional Epithelium: A type of epithelium that can stretch and is found in the bladder and ureters.

Microvilli: Tiny projections on epithelial cells that increase surface area for absorption, found in the small intestine.

 

Instructions

This is the handout portion of the Tissue lab.  Using the PPT shared with you, complete the handout given to you by your instructor. You will need to copycat 5 different slides, working with a partner. Only choose slides of structures you have access to. Make sure your labeled slides have at least 3 different structures labeled. Remember to indicate with slide # you are mimicking in the sample slides. Do not forget to include the total magnification on your 5 slides. Although you are working in pairs, each student will need to upload his or her own PPT.  Follow the procedure below:

1. Select 5 different types of labeled histology slides to view that you’ve been provided as examples to study: Choose from the following categories:

Blood Vessels

Kidney Structures

Lung Tissue

Trachea

Small Intestine

Esophagus

Uterus

Fallopian Tube

Vagina

Colon

 

2. Examine each slide carefully: Look for key features and labels associated with the different tissues.

3. Label the slides: Using a clear labeling method, identify the following structures on each slide:

•      Type of Epithelium (e.g., simple squamous, simple cuboidal)
•      Key Features (e.g., lumen, blood vessel layers, goblet cells)
•      Any other specific structures as indicated in your example

4. Write a Brief Description: For each slide, write a short description (2-3 sentences) explaining the significance of the identified structures and their functions in the body. This should go in the ‘Notes’ section of your PowerPoint.

5. Submit Your Work: Once all slides are labeled and descriptions are completed, submit your labeled slides and descriptions.

Post Lab Histology Questions

 

1. Describe the primary function of simple squamous epithelium and where it can be found in the body.
2. .What is the main role of the tunica media in blood vessels?
3. Identify the type of epithelium found in the kidney tubules.
4. Explain the significance of the lumen in blood vessels.
5. What distinguishes the cortex from the medulla in kidney tissue?
6. Define the term “renal corpuscle”. What are its components?
7. What is the role of goblet cells in the digestive system?
8. How does the structure of the alveolus facilitate gas exchange?
9. What are the three major layers of blood vessels, and what are their functions?
10. Describe the appearance of transitional epithelium and where it is typically located.

Multiple Choice Questions. Choose the correct answer for each question below

11.Which type of epithelium lines the alveoli in the lungs?
A) Simple cuboidal
B) Stratified squamous
C) Simple squamous
D) Pseudostratified columnar

12.What is the function of the basement membrane in epithelial tissues?
A) Provides structural support
B) Facilitates nutrient absorption
C) Protects against pathogens
D) All of the above

13.Which of the following vessels contains a tunica adventitia?
A) Capillary
B) Arteriole
C) Venule
D) All of the above

14. What type of cells are primarily found in the fallopian tubes?
A) Stratified squamous
B) Simple cuboidal
C) Simple columnar
D) Transitional

15. What is the primary function of the serosa in the small intestine?
A) Absorption
B) Protection and lubrication
C) Filtration
D) Secretion

16. Which histological feature is characteristic of the respiratory epithelium?
A) Microvilli
B) Cilia
C) Goblet cells
D) Both B and C

17. What type of epithelium is found in the esophagus?
A) Simple cuboidal
B) Stratified squamous
C) Pseudostratified columnar
D) Transitional

18. Which structure in the kidney is responsible for filtration?
A) Collecting duct
B) Glomerulus
C) Renal tubule
D) Renal corpuscle

19. In which part of the body would you find keratinized stratified squamous epithelium?
A) Lining of the stomach
B) Skin
C) Lining of the bladder
D) Alveoli

20. Which layer of the bladder epithelium is adapted for stretching?
A) Basal cells
B) Umbrella cells
C) Transitional cells
D) Both B and C